Plate Count Agar

Plate Count Agar is an essential medium for the cultivation of bacteria and the enumeration of microorganisms in various samples, including water, sewage, and feces. Formulated to meet the highest standards, our agar is compliant with guidelines from APHA, PHLS, and ISO for accurate microbial counts in food safety testing and environmental monitoring.

Key Ingredients:

Pancreatic Digest of Casein (5.0 g/l): Provides vital amino acids and nitrogenous compounds essential for robust bacterial growth.

Yeast Extract (2.5 g/l): Enriches the medium with Vitamin B complex, promoting the growth of diverse microbial populations.

Dextrose (1.0 g/l): Supplies necessary sugars for enhanced microbial proliferation.

Agar (15.0 g/l): Acts as a solidifying agent, forming a stable gel for reliable colony counting.

Preparation Instructions:

To prepare the medium, suspend 23.5 g of Plate Count Agar in 1 liter of deionized or distilled water. Heat with frequent agitation until fully dissolved, then boil for 1 minute. Dispense into sterile tubes or flasks and sterilize in an autoclave at 121°C (15 lbs.) for 15 minutes.

Principle and Use:

Plate Count Agar serves as the go-to medium for the plate count of microorganisms in food and other materials. Following the APHA pour plate method, diluted samples are added to Petri plates before sterile molten agar is introduced, ensuring even mixing. This method provides higher recovery rates compared to surface inoculation, making it ideal for applications in sterile environments and laboratories.

Physical Appearance:

Our dehydrated Plate Count Agar appears as a free-flowing yellowish powder, which reconstitutes into a transparent yellowish gel upon preparation.

Storage and Shelf Life:

Store tightly capped in the original container at 5-30°C. The dehydrated medium boasts a shelf life of 3 years from the manufacturing date, while the prepared medium can be stored away from light at 2-8°C.

Quality Control:

Cultural characteristics should be evaluated after incubation at 35-37°C for 18-48 hours, ensuring optimal performance for microbial analysis.

Why Choose Plate Count Agar?

Our Plate Count Agar is not just a laboratory tool; it’s a critical component in ensuring food safety and monitoring environmental quality. By facilitating accurate bacterial enumeration, this medium supports your efforts in achieving the highest standards of public health and safety.

Microorganism	Strains Number	Inoculum (CFU)	Growth	Recovery	Remarks
Pseudomonas aeruginosa	ATCC 9027	20-200	luxuriant	≥70%	Colorless transparent colonies
Escherichia coli	ATCC 25922	20-200	luxuriant	≥70%	Colorless transparent large colonies
Bacillus subtilis	ATCC 6633	20-200	luxuriant	≥70%	White irregular colonies
Bacillus cereus	ATCC 11778	20-200	luxuriant	≥70%	White irregular colonies
Staphylococcus Aureus	ATCC 6538	20-200	luxuriant	≥70%	Yellowish colonies

Product advantage

Dissolves quickly without clumping.

No dust, more environmentally-friendly, biosafety compliance.

Not easy to absorb moisture, easier to weigh.

More stable mass.

Bagged granular medium is easy to use without weighing.

RFQ

Q: How long is your delivery time?

A: Usually it takes about 7 working days after the deposit received, it depends on the quantity.

Q: Do you provide samples ? is it free or extra ?

A: Yes, we could offer samples of most products for free charge but do not pay the cost of freight.

Q: How to get quotation?

A: Please tell us the the products and the quantites you need, we will offer you our best prices.

Q: Can you design for us?

A: Of course, we accept various OEM and ODM orders.

Q: How do I pay?

A: You can pay us by wire transfer, bank transfer, PayPal, etc.

Q: How many kinds of products do you have?

A: As many as 1000 kinds, which makes Hopebio to be one of the manufacturers which have the most complete microbial products series in China.